Heuristic exploration of the relationship between cases and viral load
Marlin Lee
1/28/2022
## [1] "MMSD-P2" "MMSD-P7" "MMSD-P8" "MMSD-P11" "MMSD-P18" "Madison"1 Data: The first look
The two data sets used in this analysis are the Madison case data sourced from the Wisconsin DHS and wastewater concentration data produced by the Wisconsin State Laboratory of Hygiene. This wastewater data has entries every couple of days from 25 January 2021 to 04 January 2022.
| Date | Site | FirstConfirmed | SevenDayMACases | N1 | BCoV | N2 | PMMoV | GeoMeanN12 | FlowRate | temperature | equiv_sewage_amt |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2021-01-25 | MMSD-P11 | 8 | 10.571429 | 280459 | 8.99 | 354015 | 31434596 | 315097.91 | 9.31 | NA | 1 |
| 2021-01-25 | MMSD-P18 | 17 | 8.571429 | 310278 | 10.37 | 447153 | 29445680 | 372480.52 | 11.82 | NA | 1 |
| 2021-01-25 | MMSD-P2 | 13 | 9.571429 | 62166 | 11.38 | 94675 | 35099462 | 76717.44 | 5.74 | NA | 1 |
| 2021-01-25 | MMSD-P7 | 5 | 10.000000 | 64345 | 10.95 | 107125 | 22900169 | 83023.84 | 4.35 | NA | 1 |
| 2021-01-25 | MMSD-P8 | 4 | 12.000000 | 66705 | 11.63 | 110510 | 22716404 | 85857.85 | 6.06 | NA | 1 |
| 2021-01-28 | MMSD-P11 | 19 | 24.857143 | 1107390 | 1.98 | 1098130 | 24862163 | 1102750.28 | 9.07 | NA | 1 |
The case data has a strong weekend effect so for this section we look at a seven day smoothing of cases. The simple display of the data shows the core components of this story. First, wastewater data is noisy. And that there is a clear relationship between the two signals.
FirstImpresionFunc <- function(DF){
FirstImpressionDF <- DF%>%
NoNa(SelectedIndVar,SelectedDepVar)#Removing NA
FirstImpressionGGplot = FirstImpressionDF%>%
ggplot(aes(x=Date))+#Data depends on time
geom_point(aes(y=!!sym(SelectedDepVar), color=SelectedDepVar,info=!!sym(SelectedDepVar)),size = 1)+
geom_line(aes(y=MinMaxFixing(!!sym(SelectedIndVar),!!sym(SelectedDepVar)),
color=SelectedIndVar,
info=!!sym(SelectedIndVar)))+#compares SelectedIndVar to Cases
geom_line(aes(y=(SevenDayMACases),
color="Seven Day MA Cases",
info=SevenDayMACases))+
labs(y="Reported cases")+
facet_wrap(~Site)
return(ggplotly(FirstImpressionGGplot))
}
FirstImpresionFunc(filter(FullDF,Site=="Madison"))FirstImpresionFunc(filter(FullDF,Site=="MMSD-P2"))FirstImpresionFunc(filter(FullDF,Site=="MMSD-P7"))FirstImpresionFunc(filter(FullDF,Site=="MMSD-P8"))FirstImpresionFunc(filter(FullDF,Site=="MMSD-P11"))FirstImpresionFunc(filter(FullDF,Site=="MMSD-P18"))2 Removing potential outliers
Looking at the wastewater measurements we observe there were some points many times larger than adjacent values hinting at them being outliers. We used the adjacent 10 values on each side and marked points 2.5 standard deviations away from the group mean as outliers.
OutlierRemoveFunc <- function(DF){
ErrorMarkedDF <- DF%>%#
mutate(FlagedOutliers = IdentifyOutliers(!!sym(SelectedIndVar),Bin = 21, Action = "Flag"),
#Manual flagging that method misses due to boundary effect with binning
FlagedOutliers = ifelse(Date == mdy("01/26/2021") | Date == mdy("01/27/2021"),
TRUE, FlagedOutliers),
NoOutlierVar = ifelse(FlagedOutliers, NA, !!sym(SelectedIndVar)))
return(ErrorMarkedDF)
}
MadDF <- OutlierRemoveFunc(filter(FullDF,Site=="Madison"))
P2DF <- OutlierRemoveFunc(filter(FullDF,Site=="MMSD-P2"))
P7DF <- OutlierRemoveFunc(filter(FullDF,Site=="MMSD-P7"))
P8DF <- OutlierRemoveFunc(filter(FullDF,Site=="MMSD-P8"))
P11DF <- OutlierRemoveFunc(filter(FullDF,Site=="MMSD-P11"))
P18DF <- OutlierRemoveFunc(filter(FullDF,Site=="MMSD-P18"))
OutlierPlotFunc <- function(DF){
#Split N1 into outlier and non outlier for next ggplot
OutLierPlotDF <- DF%>%
mutate(!!OutlierName := ifelse(FlagedOutliers,!!sym(SelectedIndVar),NA))%>%
mutate(!!SelectedIndVar := NoOutlierVar)
OutLierPlotObject <- OutLierPlotDF%>%
filter(!(is.na(!!sym(SelectedIndVar))&is.na(!!sym(OutlierName))))%>%
ggplot(aes(x=Date))+#Data depends on time
geom_line(aes(y=!!sym(SelectedIndVar),
color=SelectedIndVar,
info = !!sym(SelectedIndVar)))+#compares Var to Cases
geom_point(aes(y=!!sym(OutlierName),
color=OutlierName,
info = !!sym(OutlierName)))+
ColorRule+
facet_wrap(~Site)
#mentioned hand picked list other choices
ReturnPlot <- ggplotly(OutLierPlotObject,tooltip=c("info","Date"))%>%
layout(yaxis = SecondAxisFormat,
legend=list(title=list(text=''),x = 1.15, y = 0.9),
margin = list(l = 50, r = 75, b = 50, t = 50, pad = 4))
return(ReturnPlot)
}
OutlierPlotFunc(MadDF)OutlierPlotFunc(P2DF)OutlierPlotFunc(P7DF)OutlierPlotFunc(P8DF)OutlierPlotFunc(P11DF)OutlierPlotFunc(P18DF)PrepOutlierFunc <- function(DF){
#Drop Var create Var filter
UpdatedDF <- DF%>%
select(-sym(SelectedIndVar))%>%
rename(!!sym(SelectedIndVar) := NoOutlierVar)
return(UpdatedDF)
}
#MadDF <- PrepOutlierFunc(MadDF)
#P2DF <- PrepOutlierFunc(P2DF)
#P7DF <- PrepOutlierFunc(P7DF)
#P8DF <- PrepOutlierFunc(P8DF)
#P11DF <- PrepOutlierFunc(P11DF)
#P18DF <- PrepOutlierFunc(P18DF)
#FullDF
DetectedMain <- MadDF%>%
filter(FlagedOutliers)%>%
select(Date,FlagedOutliers)
StevePlot <- FullDF%>%
ggplot()+
aes(x=Date)+
geom_vline(aes(xintercept=Date),color="red",data=DetectedMain)+
geom_point(aes(y=N1))+
facet_wrap(~Site, scale = "free_y")
StevePlot
A <- MadDF%>%
filter(FlagedOutliers)
B <- P2DF%>%
filter(FlagedOutliers)
C <- P7DF%>%
filter(FlagedOutliers)
D <- P8DF%>%
filter(FlagedOutliers)
E <- P11DF%>%
filter(FlagedOutliers)
fF <- P18DF%>%
filter(FlagedOutliers)
AllErrors <- full_join(full_join(full_join(full_join(full_join(A,B),C),D),E),fF)
AllErrors## Date Site FirstConfirmed SevenDayMACases N1 BCoV N2
## 1 2021-01-26 Madison 56 89.428571 1831813 6.930 1686654
## 2 2021-01-27 Madison 162 88.857143 1357329 2.059 1323010
## 3 2021-04-15 Madison 56 50.285714 571269 3.684 706418
## 4 2021-04-26 Madison NA 39.333333 675748 16.079 546554
## 5 2021-05-02 Madison 26 37.333333 876165 4.710 1134521
## 6 2021-05-14 Madison 37 23.500000 324203 5.070 465104
## 7 2021-06-06 Madison NA 8.000000 658991 9.570 647639
## 8 2021-06-08 Madison 8 8.000000 1459947 0.982 1464354
## 9 2021-07-26 Madison 24 43.333333 186460 3.589 143034
## 10 2021-07-28 Madison 62 48.428571 260334 27.638 207880
## 11 2021-10-17 Madison 58 56.428571 823463 1.686 693348
## 12 2021-11-26 Madison 55 104.600000 1705574 6.299 1758779
## 13 2021-01-26 MMSD-P2 22 16.428571 NA NA NA
## 14 2021-01-27 MMSD-P2 48 26.714286 NA NA NA
## 15 2021-01-26 MMSD-P7 3 18.428571 NA NA NA
## 16 2021-01-27 MMSD-P7 15 28.142857 NA NA NA
## 17 2021-01-26 MMSD-P8 7 23.428571 NA NA NA
## 18 2021-01-27 MMSD-P8 24 25.428571 NA NA NA
## 19 2021-01-26 MMSD-P11 13 10.000000 NA NA NA
## 20 2021-01-27 MMSD-P11 56 24.142857 NA NA NA
## 21 2021-01-26 MMSD-P18 10 9.142857 NA NA NA
## 22 2021-01-27 MMSD-P18 18 24.428571 NA NA NA
## PMMoV GeoMeanN12 FlowRate temperature equiv_sewage_amt FlagedOutliers
## 1 29748189 1757735.7 37.74 NA 1.000 TRUE
## 2 30102158 1340059.6 36.17 NA 1.000 TRUE
## 3 23628196 635259.6 39.02 NA 1.000 TRUE
## 4 28619982 607727.5 36.04 NA 0.250 TRUE
## 5 42099107 997009.3 35.23 NA 0.250 TRUE
## 6 34292122 388314.5 36.12 NA 0.625 TRUE
## 7 32185831 653290.3 34.39 NA 1.000 TRUE
## 8 54673826 1462148.8 36.40 NA 1.000 TRUE
## 9 30230924 163309.9 34.93 NA 1.000 TRUE
## 10 37320637 232633.3 36.82 NA 1.000 TRUE
## 11 29427968 755610.0 35.38 NA 0.625 TRUE
## 12 26466528 1731972.2 32.48 NA 0.625 TRUE
## 13 NA NA NA NA NA TRUE
## 14 NA NA NA NA NA TRUE
## 15 NA NA NA NA NA TRUE
## 16 NA NA NA NA NA TRUE
## 17 NA NA NA NA NA TRUE
## 18 NA NA NA NA NA TRUE
## 19 NA NA NA NA NA TRUE
## 20 NA NA NA NA NA TRUE
## 21 NA NA NA NA NA TRUE
## 22 NA NA NA NA NA TRUE
## NoOutlierVar
## 1 NA
## 2 NA
## 3 NA
## 4 NA
## 5 NA
## 6 NA
## 7 NA
## 8 NA
## 9 NA
## 10 NA
## 11 NA
## 12 NA
## 13 NA
## 14 NA
## 15 NA
## 16 NA
## 17 NA
## 18 NA
## 19 NA
## 20 NA
## 21 NA
## 22 NA#On a given day we have 5 intercepters
OutlierPlotFunc(MadDF)OutlierPlotFunc(P2DF)OutlierPlotFunc(P7DF)OutlierPlotFunc(P8DF)OutlierPlotFunc(P11DF)OutlierPlotFunc(P18DF)AllErrors%>%
ggplot()+
aes(x=Date)+
geom_point(aes(y=N1,color=Site),size=2)+
geom_vline(xintercept = ymd("2021-04-26"))+
geom_vline(xintercept = ymd("2021-11-18"))+
facet_wrap(~Site, scale = "free_y")
AllErrors%>%
filter(!is.na(N1))%>%
group_by(Date)%>%
summarize(n())## # A tibble: 12 x 2
## Date `n()`
## <date> <int>
## 1 2021-01-26 1
## 2 2021-01-27 1
## 3 2021-04-15 1
## 4 2021-04-26 1
## 5 2021-05-02 1
## 6 2021-05-14 1
## 7 2021-06-06 1
## 8 2021-06-08 1
## 9 2021-07-26 1
## 10 2021-07-28 1
## 11 2021-10-17 1
## 12 2021-11-26 1#"2021-01-26"
#"2021-06-06"
#"2021-06-08"
#"2021-10-17"
#"2021-11-26"
"2021-04-26"## [1] "2021-04-26""2021-11-18"## [1] "2021-11-18"#P2 error seen in madison: maybe?
#P7 error seen in madison: maybe?
#P8 error seen in madison: no
#P8 error seen in madison: no
#P18 error seen in madison: yes, sometimesLoessPlotFunc <- function(DF,SpanConstant = .163){
MainDF <- DF
MainDF[[loessVar]] <- loessFit(y=(MainDF[[SelectedIndVar]]),
x=MainDF$Date, #create loess fit of the data
span=SpanConstant, #span of .2 seems to give the best result, not rigorously chosen
iterations=2)$fitted#2 iterations remove some bad patterns
MainDF <- MainDF%>%
NoNa(loessVar,"SevenDayMACases")
SLDLoessGraphic <- MainDF%>%
ggplot(aes(x=Date))+
geom_line(aes(y=!!sym(SelectedDepVar), color=SelectedDepVar , info = !!sym(SelectedDepVar)),alpha=.1)+
geom_line(aes(y=MinMaxFixing(!!sym(SelectedIndVar),!!sym(SelectedDepVar)),
color=SelectedIndVar,
info = !!sym(SelectedIndVar)),
alpha=.2)+
geom_line(aes(y=SevenDayMACases,
color="Seven Day MA Cases" ,
info = SevenDayMACases))+
geom_line(aes(y=MinMaxFixing(!!sym(loessVar),!!sym(SelectedDepVar),!!sym(SelectedIndVar)),
color=loessVar ,
info = !!sym(loessVar)))+
facet_wrap(~Site)+
labs(y="Reported cases")
ReturnPlot <- ggplotly(SLDLoessGraphic,tooltip=c("info","Date"))%>%
add_lines(x=~Date, y=MainDF[[SelectedIndVar]],
yaxis="y2", data=MainDF, showlegend=FALSE, inherit=FALSE) %>%
layout(yaxis2 = SecondAxisFormat,
legend=list(title=list(text=''),x = 1.15, y = 0.9),
margin = list(l = 50, r = 75, b = 50, t = 50, pad = 4))
return(ReturnPlot)
}
LoessPlotFunc(MadDF)LoessPlotFunc(P2DF)LoessPlotFunc(P7DF)LoessPlotFunc(P8DF)LoessPlotFunc(P11DF)LoessPlotFunc(P18DF)OutlierDF <- MadDF%>%
filter(FlagedOutliers)
FullDFMassRatio <- FullDF%>%
filter(!is.na(N1))%>%
mutate(SC2_mass = (3.785*1e6)*FlowRate*N1)
MissingIntercepter <- FullDFMassRatio%>%
filter(Site!="Madison",!is.na(FlowRate)|!is.na(N1))%>%
group_by(Date)%>%
summarize(N = n())%>%
filter(N!=5)
FullDFMassRatio.Mad <- FullDFMassRatio%>%
filter(!is.na(SC2_mass))%>%
filter(Site=="Madison")
FullDFMassRatio.Inter <- FullDFMassRatio%>%
filter(!is.na(SC2_mass))%>%
filter(Site!="Madison")#TempErrorMetric
TempErrorMetric <- FullDFMassRatio.Inter%>%
group_by(Date)%>%
summarise(InterSum = sum(SC2_mass),InterSumFlow = sum(FlowRate))%>%
inner_join(FullDFMassRatio.Mad)%>%
mutate(MassRatio = SC2_mass/InterSum,
ErrorRatio = 2*(SC2_mass-InterSum)/(SC2_mass+InterSum),
MassRatioFlow = FlowRate/InterSumFlow,
ErrorRatioFlow = 2*(FlowRate-InterSumFlow)/(FlowRate+InterSumFlow))
FullDFMassRatio.Mad <- FullDFMassRatio%>%
filter(!is.na(SC2_mass))%>%
filter(Site=="Madison")%>%
inner_join(FullDFMassRatio.Inter["Date"])
FullDFMassRatio.Inter <- FullDFMassRatio%>%
filter(Site!="Madison")TempErrorMetric%>%
summarise(MeanError = mean(ErrorRatio, na.rm=TRUE),
MeanRatio = mean(MassRatio, na.rm=TRUE),
MedianError = median(ErrorRatio, na.rm=TRUE),
MedianRatio = median(MassRatio, na.rm=TRUE),
n())## # A tibble: 1 x 5
## MeanError MeanRatio MedianError MedianRatio `n()`
## <dbl> <dbl> <dbl> <dbl> <int>
## 1 -0.0384 1.18 -0.0313 0.969 93ToMergeDF <- TempErrorMetric[c("Date","ErrorRatio","MassRatio","MassRatioFlow","ErrorRatioFlow")]
MadErrorRatioDF <- full_join(ToMergeDF,MadDF)%>%
filter(!is.na(N1),!is.na(MassRatio),!is.na(MassRatio))
MadErrorRatioFilledDF <- MadErrorRatioDF%>%
setorder(Date)%>%
fill(ErrorRatio,MassRatio,.direction ="down")
MadErrorRatioDF%>%
filter(!is.na(ErrorRatio))%>%
group_by(FlagedOutliers)%>%
summarise(MeanError = mean(ErrorRatio, na.rm=TRUE),
MeanRatio = mean(MassRatio, na.rm=TRUE),
MedianError = median(ErrorRatio, na.rm=TRUE),
MedianRatio = median(MassRatio, na.rm=TRUE),
n())## # A tibble: 2 x 6
## FlagedOutliers MeanError MeanRatio MedianError MedianRatio `n()`
## <lgl> <dbl> <dbl> <dbl> <dbl> <int>
## 1 FALSE -0.0700 1.05 -0.0352 0.965 90
## 2 TRUE 0.909 4.87 1.01 3.02 3MadErrorRatioFilledDF%>%
filter(!is.na(ErrorRatio))%>%
group_by(FlagedOutliers)%>%
summarise(MeanError = mean(ErrorRatio, na.rm=TRUE),
MeanRatio = mean(MassRatio, na.rm=TRUE),
MedianError = median(ErrorRatio, na.rm=TRUE),
MedianRatio = median(MassRatio, na.rm=TRUE),
n())## # A tibble: 2 x 6
## FlagedOutliers MeanError MeanRatio MedianError MedianRatio `n()`
## <lgl> <dbl> <dbl> <dbl> <dbl> <int>
## 1 FALSE -0.0700 1.05 -0.0352 0.965 90
## 2 TRUE 0.909 4.87 1.01 3.02 3HighRatioVec <- ToMergeDF%>%
filter(MassRatio>1.5)
MadErrorRatioFilledDF%>%
filter(FlagedOutliers)## # A tibble: 3 x 18
## Date ErrorRatio MassRatio MassRatioFlow ErrorRatioFlow Site
## <date> <dbl> <dbl> <dbl> <dbl> <chr>
## 1 2021-04-15 1.65 10.5 1.77 0.557 Madison
## 2 2021-04-26 0.0706 1.07 1 0 Madison
## 3 2021-07-26 1.01 3.02 1 0 Madison
## # ... with 12 more variables: FirstConfirmed <int>, SevenDayMACases <dbl>,
## # N1 <int>, BCoV <dbl>, N2 <int>, PMMoV <dbl>, GeoMeanN12 <dbl>,
## # FlowRate <dbl>, temperature <dbl>, equiv_sewage_amt <dbl>,
## # FlagedOutliers <lgl>, NoOutlierVar <int>MadErrorRatioDF%>%
ggplot()+
aes(x=MassRatio,y=N1,color = FlagedOutliers)+
geom_point(size=5,alpha=.5)+
scale_y_log10()+
scale_x_log10()+
ggtitle("FlagedOutlier vs N1")
MadErrorRatioFilledDF%>%
ggplot()+
aes(x=MassRatio,y=N1,color = FlagedOutliers)+
geom_point(size=5,alpha=.5)+
scale_y_log10()+
scale_x_log10()+
ggtitle("FlagedOutlier vs N1 Filled Data")
t.test(ErrorRatio ~ FlagedOutliers, data = MadErrorRatioFilledDF)##
## Welch Two Sample t-test
##
## data: ErrorRatio by FlagedOutliers
## t = -2.1197, df = 2.0507, p-value = 0.165
## alternative hypothesis: true difference in means between group FALSE and group TRUE is not equal to 0
## 95 percent confidence interval:
## -2.9211455 0.9622938
## sample estimates:
## mean in group FALSE mean in group TRUE
## -0.06998153 0.90944431#MadErrorRatioFilledDF
#MadErrorRatioDF
MadErrorRatioDF%>%
ggplot()+
aes(x=MassRatioFlow,y=MassRatio,color=FlagedOutliers)+
geom_point(size=5,alpha=.5)+
ggtitle("FlowRatio vs MassFlow")
MadErrorRatioDF%>%
filter(MassRatioFlow==1)## # A tibble: 73 x 18
## Date ErrorRatio MassRatio MassRatioFlow ErrorRatioFlow Site
## <date> <dbl> <dbl> <dbl> <dbl> <chr>
## 1 2021-01-25 -0.370 0.688 1 0 Madison
## 2 2021-01-28 -0.535 0.578 1 0 Madison
## 3 2021-02-01 -0.503 0.598 1 0 Madison
## 4 2021-02-04 0.463 1.60 1 0 Madison
## 5 2021-02-08 -0.655 0.507 1 0 Madison
## 6 2021-02-11 0.719 2.12 1 0 Madison
## 7 2021-02-15 -0.174 0.840 1 0 Madison
## 8 2021-02-18 -0.236 0.789 1 0 Madison
## 9 2021-02-25 0.0805 1.08 1 0 Madison
## 10 2021-03-01 0.118 1.13 1 0 Madison
## # ... with 63 more rows, and 12 more variables: FirstConfirmed <int>,
## # SevenDayMACases <dbl>, N1 <int>, BCoV <dbl>, N2 <int>, PMMoV <dbl>,
## # GeoMeanN12 <dbl>, FlowRate <dbl>, temperature <dbl>,
## # equiv_sewage_amt <dbl>, FlagedOutliers <lgl>, NoOutlierVar <int>KeyOulierPoints <- c(ymd("2021-06-08"),
ymd("2021-10-17"),
ymd("2021-05-02"),
ymd("2021-01-26"))
OutLierPlotDF <- MadDF%>%
mutate(Outlier = ifelse(FlagedOutliers,N1,NA))%>%
mutate(N1 = NoOutlierVar)%>%
filter(!(is.na(N1)&is.na(Outlier)))%>%
ggplot(aes(x=Date))+#Data depends on time
geom_line(aes(y=N1,
color="N1",
info = !!sym(SelectedIndVar)))+#compares Var to Cases
geom_point(aes(y=Outlier,
color="N1 Outlier",
info = Outlier))+
scale_color_manual(values=c("#F8766D","#999999")) #+
#geom_vline(xintercept = KeyOulierPoints)+
#geom_vline(xintercept = MissingIntercepter$Date,color="Red")+
facet_wrap(~Site)## <ggproto object: Class FacetWrap, Facet, gg>
## compute_layout: function
## draw_back: function
## draw_front: function
## draw_labels: function
## draw_panels: function
## finish_data: function
## init_scales: function
## map_data: function
## params: list
## setup_data: function
## setup_params: function
## shrink: TRUE
## train_scales: function
## vars: function
## super: <ggproto object: Class FacetWrap, Facet, gg>ggplotly(OutLierPlotDF)#"2021-06-08","2021-10-17","2021-05-02","2021-01-26"FlowPlot <- FullDFMassRatio.Inter%>%
filter(!is.na(FlowRate))%>%
ggplot()+
geom_bar(aes(x=Date,y=FlowRate,fill=Site),position="stack", stat="identity", width = 3) +
scale_fill_brewer(palette="Spectral") +
scale_color_manual(values=c("#d60000", "#ffa600", "#7ed05c", "#5c9c43", "#3a56d6")) +
theme_light() +
geom_line(data=FullDFMassRatio.Mad,aes(x=Date,y=FlowRate),size=.5)+
ylab("Average daily flow (MGD)") +
geom_hline(yintercept=median(FullDFMassRatio.Mad$FlowRate), linetype = "dashed", color="black") +
#geom_vline(xintercept = KeyOulierPoints)+
#geom_vline(xintercept = MissingIntercepter$Date,color="Red")+
ggtitle("Average daily flow (MMSD)")
ggplotly(FlowPlot)MassBalencePlot <- FullDFMassRatio.Inter%>%
filter(!is.na(FlowRate))%>%
ggplot()+
geom_bar(aes(x=Date,y=SC2_mass,fill=Site),position="stack", stat="identity", width = 3) +
scale_fill_brewer(palette="Spectral") +
scale_color_manual(values=c("#d60000", "#ffa600", "#7ed05c", "#5c9c43", "#3a56d6")) +
theme_light() +
geom_point(data=FullDFMassRatio.Mad,aes(x=Date,y=SC2_mass),size=1)+
ylab("N1/N2 gene copies per day") +
geom_hline(yintercept=median(FullDFMassRatio.Mad$SC2_mass), color="red") +
ggtitle("SARS-CoV-2, 24h Mass Loading (Madison)")
ggplotly(MassBalencePlot)MassBalencePlot <- FullDFMassRatio.Inter%>%
filter(!is.na(FlowRate))%>%
ggplot()+
geom_bar(aes(x=Date,y=FirstConfirmed,fill=Site),position="stack", stat="identity", width = 3) +
scale_fill_brewer(palette="Spectral") +
scale_color_manual(values=c("##29ffff", "#0059ff", "#812fa3", "#a363bc", "#c5a929")) +
theme_light() +
geom_point(data=FullDFMassRatio.Mad,aes(x=Date,y=FirstConfirmed),size=1)+
ylab("N1/N2 gene copies per day") +
geom_hline(yintercept=median(FullDFMassRatio.Mad$FirstConfirmed), color="red") +
ggtitle("Cases, 24h Mass Loading (Madison)")
ggplotly(MassBalencePlot)#ggplotly(OutLierPlotDF)
#ggplotly(MassBalencePlot)
#ggplotly(FlowPlot)
#OutlierDF
a <- OutLierPlotDF+
geom_vline(xintercept = OutlierDF$Date)+
#geom_vline(xintercept = MissingIntercepter$Date,color="Red")+
theme(title = element_blank(),
axis.text.x = element_blank(),
axis.title.x = element_blank())
b <- MassBalencePlot+
geom_vline(xintercept = OutlierDF$Date)+
#geom_vline(xintercept = MissingIntercepter$Date,color="Red")+
theme(title = element_blank(),
axis.text.x = element_blank(),
axis.title.x = element_blank())
c <- FlowPlot+
geom_vline(xintercept = OutlierDF$Date)+
#geom_vline(xintercept = MissingIntercepter$Date,color="Red")+
theme(title = element_blank(),
axis.text.x = element_blank(),
axis.title.x = element_blank())
library(ggpubr)
ggarrange(a,b,c,nrow=3,align ="v")
#FullDFMassRatio.Inter%>%
# filter(Date==ymd("2021-04-15"))
#MMSD-P18/P7
#2021-04-15
a
ggarrange(a,b,nrow=3,align ="v")